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Anti-AQP9 Polyclonal Antibody (HT226014)

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Overview
Catalog No.HT226014
Description
Anti-AQP9 Polyclonal Antibody (HT226014) is a rabbit polyclonal antibody detecting AQP9 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human AQP9 (Tyr125-Leu158).
Target AQP-9, SSC1, Small solute channel 1, Aquaporin-9, AQP9, Aquaglyceroporin-9
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O43315
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Aquaporin-9 (AQP9) is a ~31 kDa protein. Aquaglyceroporins form homotetrameric transmembrane channels, with each monomer independently mediating glycerol and water transport across the plasma membrane along their osmotic gradient. AQP9 is the primary route for glycerol uptake in hepatocytes, supporting hepatic gluconeogenesis. It exhibits broad specificity and may transport various small, non-charged solutes, including carbamides, polyols, purines, and pyrimidines. AQP9 may also facilitate hepatic urea extrusion. Due to its permeability to lactate, AQP9 might participate in the astrocyte-to-neuron lactate shuttle, supplying neurons with energy.

1. Tsukaguchi, H. et al. (1999) The American journal of physiology 277, F685-96. PMID: 10564231
2. Gotfryd, K. et al. (2018) Nature communications 9, 4749. PMID: 30420639
3. Geistlinger, K. et al. (2022) Life (Basel, Switzerland) 12. PMID: 35054513
4. Ishibashi, K. et al. (1998) Biochemical and biophysical research communications 244, 268-74. PMID: 9514918
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-AQP9 Polyclonal Antibody

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