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Anti-Arabidopsis thaliana CARK1 Polyclonal Antibody (PR011014)

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Overview
Catalog No.PR011014
Description
Anti-Arabidopsis thaliana CARK1 Polyclonal Antibody (PR011014) is a rabbit polyclonal antibody detecting CARK1. Suitable for Arabidopsis thaliana.
Species reactivityArabidopsis thaliana (Mouse-ear cress)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Arabidopsis thaliana CARK1 (Gln50-Asn350).
Target CARK1, EC:2.7.11.1, Protein CYTOSOLIC ABA RECEPTOR KINASE 1, Receptor-like cytoplasmic kinase 1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9LUT0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Protein CYTOSOLIC ABA RECEPTOR KINASE 1 (CARK1) is a ~39 kDa protein. Receptor-like cytoplasmic kinase (RLCK) that triggers abscisic acid (ABA) signaling in response to dehydration by phosphorylating and activating ABA receptors (e.g. PYL8/RCAR3 and PYR1/RCAR11) to support their monomerization and enhance their ABA binding affinity; they in turn repress PP2C protein phosphatases (e.g. ABI1) acting as negative regulators of ABA responses. Promotes drought tolerance.

1. Zhang, L. et al. (2018) Cell discovery 4, 30. PMID: 29928509
2. Li, X. et al. (2022) The New phytologist 235, 533-549. PMID: 35388459
3. Ge, H. et al. (2018) International journal of molecular sciences 19. PMID: 29970817
Note For research use only
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Formula
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Arabidopsis thaliana CARK1 Polyclonal Antibody

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