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Anti-ARFIP1 Polyclonal Antibody (HA647014)

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Overview
Catalog No.HA647014
Description
Anti-ARFIP1 Polyclonal Antibody (HA647014) is a rabbit polyclonal antibody detecting Arfaptin-1; ADP-ribosylation factor-interacting protein 1; ARFIP1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human ARFIP1 (Ile117-Gln373).
Target Arfaptin-1; ADP-ribosylation factor-interacting protein 1; ARFIP1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P53367, G5E8V9, Q9JHU5
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Arfaptin-1 (ARFIP1) is a ~41 kDa protein. Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network. Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold. Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs. In turn, ARFs induce fission. Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells.

1. Gehart, H. et al. (2012) Developmental cell 23, 756-68. PMID: 22981988
2. Kanoh, H. et al. (1997) The Journal of biological chemistry 272, 5421-9. PMID: 9038142
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-ARFIP1 Polyclonal Antibody

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