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Anti-ARHGEF15 Polyclonal Antibody (HB586014)

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Overview
Catalog No.HB586014
Description
Anti-ARHGEF15 Polyclonal Antibody (HB586014) is a rabbit polyclonal antibody detecting Rho guanine nucleotide exchange factor 15; Ephexin-5; E5; Vsm-RhoGEF; ARHGEF15; KIAA0915 in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human ARHGEF15 (Arg619-Thr741).
Target Rho guanine nucleotide exchange factor 15; Ephexin-5; E5; Vsm-RhoGEF; ARHGEF15; KIAA0915
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O94989, Q5FWH6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Rho guanine nucleotide exchange factor 15 (ARHGEF15) is a ~91 kDa protein. Guanine nucleotide exchange factor (GEF) that activates RhoA, playing a role in the regulation of actin cytoskeleton organization. Is involved in the regulation of vascular smooth muscle contractility. Additionally, it negatively regulates excitatory synapse development by suppressing the synapse-promoting activity of EPHB2. Does not activate RAC1 or CDC42.

1. Ding, X. et al. (2023) Acta neuropathologica 145, 681-705. PMID: 36929019
2. Ogita, H. et al. (2003) Circulation research 93, 23-31. PMID: 12775584
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-ARHGEF15 Polyclonal Antibody

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