Anti-ASAH1 Polyclonal Antibody (HC270014)

Name: Anti-ASAH1 Polyclonal Antibody
Brand: abinScience
SKU: HC270014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HC270014

Description

Anti-ASAH1 Polyclonal Antibody (HC270014) is a rabbit polyclonal antibody detecting ASAH1 in ELISA, IHC, WB. Suitable for Human, Bovine, Macaca fascicularis, and Pan troglodytes.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ASAH1 (Gln22-Trp395).

Target

PHP32, ASAH1, ACDase, AC, N-acylethanolamine hydrolase ASAH1, Acid CDase, Acylsphingosine deacylase, N-acylsphingosine amidohydrolase, Putative 32 kDa heart protein, ASAH, Acid ceramidase

Purification

Purified by antigen affinity column.

Accession

Q13510

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Acid ceramidase (ASAH1) is a ~44 kDa protein. Lysosomal ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at acidic pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Has a higher catalytic efficiency towards C12-ceramides versus other ceramides. Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine. For the reverse synthetic reaction, the natural sphingosine D-erythro isomer is more efficiently utilized as a substrate compared to D-erythro-dihydrosphingosine and D-erythro-phytosphingosine, while the fatty acids with chain lengths of 12 or 14 carbons are the most efficiently used.

1. Li, CM. et al. (1999) Genomics 62, 223-31. PMID: 10610716

2. Ferlinz, K. et al. (2001) The Journal of biological chemistry 276, 35352-60. PMID: 11451951

3. Tsuboi, K. et al. (2005) The Journal of biological chemistry 280, 11082-92. PMID: 15655246

4. Ferraz, MJ. et al. (2016) FEBS letters 590, 716-25. PMID: 26898341

5. Kumar, M. et al. (2023) Human molecular genetics 32, 1888-1900. PMID: 36752535

6. Bernardo, K. et al. (1995) The Journal of biological chemistry 270, 11098-102. PMID: 7744740

7. Yamaguchi, Y. et al. (1994) Journal of biochemistry 116, 704-10. PMID: 7852294

Note

For research use only.

Images

References

Datasheet

Anti-ASAH1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ASAH1 Polyclonal Antibody (HC270014)

Overview

Images

References

Datasheet

SDS

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