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Anti-ASFV K196R Polyclonal Antibody (VK527014)

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Overview
Catalog No.VK527014
Species reactivityAfrican swine fever virus (ASFV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant ASFV K196R (Glu35-Tyr196).
Target Thymidine kinase, 2.7.1.21, K196R, K196R CDS, ASFV-Georgia_4-074
Purification Purified by antigen affinity column.
Accession P18555
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Thymidine kinase (K196R) is a ~22 kDa protein. Phosphorylates thymidine. ASFV replicates in the cytoplasm of infected cells and contains genes encoding a number of enzymes needed for DNA synthesis, including thymidine kinase. Important for growth in swine macrophages in vitro and is a virus virulence factor in swine.

Note For research use only.
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V₁ (Stock Vol.)
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Anti-ASFV K196R Polyclonal Antibody

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