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Anti-ASFV NP868R Polyclonal Antibody (VK692014)

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Overview
Catalog No.VK692014
Description
Anti-ASFV NP868R Polyclonal Antibody (VK692014) is a rabbit polyclonal antibody detecting NP868R in ELISA, IHC, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityAfrican swine fever virus
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant ASFV NP868R (Met586-Asn868).
Target mRNA-capping enzyme, VTF/CE, Polynucleotide 5'-triphosphatase, 3.6.1.74, mRNA 5'-triphosphatase, TPase, mRNA guanylyltransferase, 2.7.7.50, GTP--RNA guanylyltransferase, GTase, mRNA (guanine-N(7))-methyltransferase, 2.1.1.56, Ba71V-101, NP868R
Purification Purified by antigen affinity column.
Accession P32094
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

mRNA-capping enzyme (NP868R) is a ~99 kDa protein. Probably catalyzes the second reaction in the mRNA cap formation pathway (Probable). Forms a covalent complex with GTP.

1. Pena, L. et al. (1993) Virology 193, 319-28. PMID: 8382399
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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