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Anti-ASS1 Polyclonal Antibody (HF801014)

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Anti-ASS1 Polyclonal Antibody
Anti-ASS1 Polyclonal Antibody
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Overview
Catalog No.HF801014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ASS1 (Glu149-Lys412).
Target ASS1, Citrulline--aspartate ligase, Argininosuccinate synthase, ASS
Purification Purified by antigen affinity column.
Accession P00966
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Argininosuccinate synthase (ASS1) is a ~46 kDa protein. One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.

Note For research use only.
Images
  • Anti-ASS1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ASS1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse kidney lysate
    Lane 2: Mouse liver lysate
    Lane 3: Rat liver lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-ASS1 Polyclonal Antibody

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