Anti-ATF7IP Polyclonal Antibody (HB362014)

Name: Anti-ATF7IP Polyclonal Antibody
Brand: abinScience
SKU: HB362014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): --

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB362014

Description

Anti-ATF7IP Polyclonal Antibody (HB362014) is a rabbit polyclonal antibody detecting Activating transcription factor 7-interacting protein 1; ATF-interacting protein; ATF-IP; ATF7-interacting protein; ATFa-associated modulator; hAM; MBD1-containing chromatin-associated factor 1; P621; ATF7IP; MCAF; MCAF1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human ATF7IP (Gln1149-Ser1270).

Target

Activating transcription factor 7-interacting protein 1; ATF-interacting protein; ATF-IP; ATF7-interacting protein; ATFa-associated modulator; hAM; MBD1-containing chromatin-associated factor 1; P621; ATF7IP; MCAF; MCAF1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q6VMQ6, Q7TT18, Q5ZIE8

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Activating transcription factor 7-interacting protein 1 (ATF7IP) is a ~136 kDa protein. Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Required for HUSH-mediated heterochromatin formation and gene silencing. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Stabilizes SETDB1, is required to stimulate histone methyltransferase activity of SETDB1 and facilitates the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3).

1. Timms, RT. et al. (2016) Cell reports 17, 653-659. PMID: 27732843

2. Fujita, N. et al. (2003) Molecular and cellular biology 23, 2834-43. PMID: 12665582

3. Wang, H. et al. (2003) Molecular cell 12, 475-87. PMID: 14536086

4. Liu, L. et al. (2009) The Journal of biological chemistry 284, 5165-74. PMID: 19106100

Note

For research use only

Images

References

Datasheet

Anti-ATF7IP Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ATF7IP Polyclonal Antibody (HB362014)

Overview

Images

References

Datasheet

SDS