Anti-ATG4B Polyclonal Antibody (HB598014)

Name: Anti-ATG4B Polyclonal Antibody
Brand: abinScience
SKU: HB598014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HB598014

Description

Anti-ATG4B Polyclonal Antibody (HB598014) is a rabbit polyclonal antibody detecting Cysteine protease ATG4B; 3.4.22.-; AUT-like 1 cysteine endopeptidase; Autophagy-related cysteine endopeptidase 1; Autophagin-1; Autophagy-related protein 4 homolog B; HsAPG4B; hAPG4B; ATG4B; APG4B; AUTL1; KIAA0943 in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human ATG4B (Met1-Leu393).

Target

Cysteine protease ATG4B; 3.4.22.-; AUT-like 1 cysteine endopeptidase; Autophagy-related cysteine endopeptidase 1; Autophagin-1; Autophagy-related protein 4 homolog B; HsAPG4B; hAPG4B; ATG4B; APG4B; AUTL1; KIAA0943

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q9Y4P1, A0A0G2QC33, Q8BGE6, Q6PZ03

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cysteine protease ATG4B is a ~44 kDa protein. Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. Required for canonical autophagy (macroautophagy), non-canonical autophagy as well as for mitophagy. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3.

1. Kabeya, Y. et al. (2004) Journal of cell science 117, 2805-12. PMID: 15169837

2. Tanida, I. et al. (2004) The Journal of biological chemistry 279, 36268-76. PMID: 15187094

3. Scherz-Shouval, R. et al. (2007) The EMBO journal 26, 1749-60. PMID: 17347651

4. Satoo, K. et al. (2009) The EMBO journal 28, 1341-50. PMID: 19322194

5. Li, M. et al. (2011) The Journal of biological chemistry 286, 7327-38. PMID: 21177865

6. Li, M. et al. (2012) Autophagy 8, 401-12. PMID: 22302004

7. Yang, Z. et al. (2015) The Journal of biological chemistry 290, 26549-61. PMID: 26378241

8. Jo, YK. et al. (2016) Oncotarget 7, 57186-57196. PMID: 27527864

9. Li, Y. et al. (2017) Autophagy 13, 1145-1160. PMID: 28633005

10. Pengo, N. et al. (2017) Nature communications 8, 294. PMID: 28821708

Note

For research use only

Images

References

Datasheet

Anti-ATG4B Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-ATG4B Polyclonal Antibody (HB598014)

Overview

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Datasheet

SDS

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