Anti-ATP2C1 Polyclonal Antibody (HX191014)

Name: Anti-ATP2C1 Polyclonal Antibody
Brand: abinScience
SKU: HX191014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HX191014

Description

Anti-ATP2C1 Polyclonal Antibody (HX191014) is a rabbit polyclonal antibody detecting ATP2C1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ATP2C1 (Val313-Ile699).

Target

Calcium-transporting ATPase type 2C member 1, ATP-dependent Ca(2+) pump PMR1, PMR1L, KIAA1347, ATP2C1, ATPase 2C1

Purification

Purified by antigen affinity column.

Accession

P98194

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Calcium-transporting ATPase type 2C member 1 (ATP2C1) is a ~100 kDa protein. ATP-driven pump that supplies the Golgi apparatus with Ca(2+) and Mn(2+) ions, both essential cofactors for processing and trafficking of newly synthesized proteins in the secretory pathway. Within a catalytic cycle, acquires Ca(2+) or Mn(2+) ions on the cytoplasmic side of the membrane and delivers them to the lumenal side. The transfer of ions across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state. Plays a primary role in the maintenance of Ca(2+) homeostasis in the trans-Golgi compartment with a functional impact on Golgi and post-Golgi protein sorting as well as a structural impact on cisternae morphology. Responsible for loading the Golgi stores with Ca(2+) ions in keratinocytes, contributing to keratinocyte differentiation and epidermis integrity.

1. Fairclough, RJ. et al. (2003) The Journal of biological chemistry 278, 24721-30. PMID: 12707275

2. Dode, L. et al. (2005) The Journal of biological chemistry 280, 39124-34. PMID: 16192278

3. Lissandron, V. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 9198-203. PMID: 20439740

4. Mukhopadhyay, S. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 858-63. PMID: 21187401

5. Chen, J. et al. (2019) The Journal of biological chemistry 294, 7878-7891. PMID: 30923126

6. Dode, L. et al. (2006) The Journal of biological chemistry 281, 3182-9. PMID: 16332677

7. Behne, MJ. et al. (2003) The Journal of investigative dermatology 121, 688-94. PMID: 14632183

Note

For research use only.

Images

References

Datasheet

Anti-ATP2C1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ATP2C1 Polyclonal Antibody (HX191014)

Overview

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References

Datasheet

SDS

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