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Anti-ATP7B Polyclonal Antibody (HW635014)

Anti-ATP7B Polyclonal Antibody
Anti-ATP7B Polyclonal Antibody
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Overview
Catalog No.HW635014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human ATP7B (Thr59-Val426).
Target Wilson disease-associated protein, Copper pump 2, WND, PWD, WC1, Copper-transporting ATPase 2, ATP7B
Purification Purified by antigen affinity column.
Accession P35670
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Copper-transporting ATPase 2 (ATP7B) is a ~157 kDa protein. Copper ion transmembrane transporter involved in the export of copper out of the cells. It is involved in copper homeostasis in the liver, where it ensures the efflux of copper from hepatocytes into the bile in response to copper overload.

Note For research use only.
Images
  • Anti-ATP7B Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ATP7B polyclonal antibody at 1ug/mL on recombinant ATP7B (Catalog No. HW635012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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