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Anti-AZIN1 Polyclonal Antibody (HT310014)

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Overview
Catalog No.HT310014
Description
Anti-AZIN1 Polyclonal Antibody (HT310014) is a rabbit polyclonal antibody detecting AZIN1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pongo abelii.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human AZIN1 (Ile5-Lys290).
Target AZIN1, OAZIN, AZI1, Antizyme inhibitor 1, OAZI, AZI, Ornithine decarboxylase antizyme inhibitor
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O14977
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Antizyme inhibitor 1 (AZIN1) is a ~49 kDa protein. Antizyme inhibitor (AZI) protein that positively regulates ornithine decarboxylase (ODC) activity and polyamine uptake. AZI is an enzymatically inactive ODC homolog that counteracts the negative effect of ODC antizymes (AZs) OAZ1, OAZ2 and OAZ3 on ODC activity by competing with ODC for antizyme-binding. Inhibits antizyme-dependent ODC degradation and releases ODC monomers from their inactive complex with antizymes, leading to formation of the catalytically active ODC homodimer and restoring polyamine production.

1. Kanerva, K. et al. (2008) The Biochemical journal 409, 187-92. PMID: 17900240
2. Wu, HY. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 11229-34. PMID: 26305948
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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