Anti-Bacillus anthracis PA/pagA/PA-83 Recombinant Antibody (SAA1376) (JN898023)

Name: Anti-Bacillus anthracis PA/pagA/PA-83 Recombinant Antibody (SAA1376)
Brand: abinScience
SKU: JN898023
Price: 126 USD
Availability: InStock

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1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

JN898023

Species reactivity

Bacillus anthracis

Applications

ELISA, WB, SPR

Host species

Human

Isotype

IgG1, kappa

Clone ID

SAA1376

Clonality

Monoclonal

Target

Protective antigen, PA, Anthrax toxins translocating protein, PA-83, PA83, Protective antigen PA-20, PA-20, PA20, Protective antigen PA-63, PA-63, PA63, pagA, pag, pXO1-110, BXA0164, GBAA_pXO1_0164

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P13423

RRID

Anti-Bacillus anthracis PA/pagA/PA-83 Antibody (SAA1376) (abinScience Cat# JN898023, RRID:AB_3726894)
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Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Protective antigen (PA/pagA) is a ~85 kDa protein. Protective antigen constitutes one of the three proteins composing the anthrax toxin; it mediates attachment to host cells and translocation of edema factor (EF) and lethal factor (LF) into the host cytoplasm. PA associated with LF forms the lethal toxin (LeTx) and causes death when injected; PA associated with EF forms the edema toxin (EdTx) and produces edema. PA induces immunity to infection with anthrax.

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1. Bradley, KA. et al. (2001) Nature 414, 225-9. PMID: 11700562

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2. Bradley, KA. et al. (2003) The Journal of biological chemistry 278, 49342-7. PMID: 14507921

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3. Santelli, E. et al. (2004) Nature 430, 905-8. PMID: 15243628

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4. Lacy, DB. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 13147-51. PMID: 15326297

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5. Singh, Y. et al. (1991) The Journal of biological chemistry 266, 15493-7. PMID: 1651334

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6. Mock, M. et al. (2001) Annual review of microbiology 55, 647-71. PMID: 11544370

Note

For research use only.

Images

Bioactivity

Detects Bacillus anthracis PA/pagA/PA-83 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Bacillus anthracis PA/pagA/PA-83 Antibody (SAA1376).
Western blot

Western blot analysis was performed using anti-Bacillus anthracis PA/pagA/PA-83 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Bacillus anthracis PA/pagA/PA-83 (Catalog No: JN898022)
Lane 2: negative control