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Anti-Bacillus cereus gajA Polyclonal Antibody (JN922014)

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Overview
Catalog No.JN922014
Description
Anti-Bacillus cereus gajA Polyclonal Antibody (JN922014) is a rabbit polyclonal antibody detecting gajA in ELISA, IHC, WB. Suitable for Bacillus cereus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityBacillus cereus
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Bacillus cereus gajA (Gly258-Gly578).
Target Endonuclease GajA, 3.1.-.-, Gabija protein GajA, Nicking endonuclease GajA, gajA, IIE_04982
Purification Purified by antigen affinity column.
Accession J8H9C1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Endonuclease GajA is a ~66 kDa protein. Component of antiviral defense system Gabija type I, composed of GajA and GajB. Endonuclease that nicks double-stranded DNA within the sequence 5'-TNNNCGGGNNA-3' in the absence of nucleotides (NTP, dNTP and NDPs), cleaving after C-1. Has no detected ATPase activity. Expression of Gabija type I in B.subtilis (strain BEST7003) confers resistance to phages phi105, phi29, rho14, SpBeta and SBSphiC. Expression of Gabija type I in E.coli B (strain ATCC 11303) confers resistance to phage T7.

1. Doron, S. et al. (2018) Science (New York, N.Y.) 359. PMID: 29371424
2. Cheng, R. et al. (2021) Nucleic acids research 49, 5216-5229. PMID: 33885789
3. Antine, SP. et al. (2024) Nature 625, 360-365. PMID: 37992757
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Bacillus cereus gajA Polyclonal Antibody

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