Anti-BCAR3 Polyclonal Antibody (HF517014)

Name: Anti-BCAR3 Polyclonal Antibody
Brand: abinScience
SKU: HF517014
Price: 168 USD
Availability: InStock

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Spec:

50ug
100ug

Number:

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References
Datasheet
SDS

Overview

Catalog No.

HF517014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human BCAR3 (Glu533-Leu825).

Target

SH2 domain-containing protein 3B, Novel SH2-containing protein 2, SH2D3B, NSP2, Breast cancer anti-estrogen resistance protein 3, BCAR3

Purification

Purified by antigen affinity column.

Accession

O75815

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Breast cancer anti-estrogen resistance protein 3 (BCAR3) is a ~92 kDa protein. Acts as an adapter protein downstream of several growth factor receptors to promote cell proliferation, migration, and redistribution of actin fibers. Specifically involved in INS/insulin signaling pathway by mediating MAPK1/ERK2-MAPK3/ERK1 activation and DNA synthesis. Promotes insulin-mediated membrane ruffling. In response to vasoconstrictor peptide EDN1, involved in the activation of RAP1 downstream of PTK2B via interaction with phosphorylated BCAR1. Inhibits cell migration and invasion via regulation of TGFB-mediated matrix digestion, actin filament rearrangement, and inhibition of invadopodia activity.

1. Oh, MJ. et al. (2013) Biochemical and biophysical research communications 441, 911-6. PMID: 24216110

2. Rufanova, VA. et al. (2009) Journal of cellular physiology 219, 45-56. PMID: 19086031

3. Oh, MJ. et al. (2008) Biochemical and biophysical research communications 375, 430-4. PMID: 18722344

4. Mace, PD. et al. (2011) Nature structural & molecular biology 18, 1381-7. PMID: 22081014

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-BCAR3 polyclonal antibody at 1ug/mL on recombinant BCAR3 (Catalog No. HF517022).

References

Datasheet

Anti-BCAR3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-BCAR3 Polyclonal Antibody (HF517014)

Overview

Images

References

Datasheet

SDS

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