Anti-BMI1 Polyclonal Antibody (HW616014)

Name: Anti-BMI1 Polyclonal Antibody
Brand: abinScience
SKU: HW616014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HW616014

Description

Anti-BMI1 Polyclonal Antibody (HW616014) is a rabbit polyclonal antibody detecting BMI1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Cat, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human BMI1 (Glu152-Ile289).

Target

RING finger protein 51, BMI1, RNF51, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, PCGF4

Purification

Purified by antigen affinity column.

Accession

P35226

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Polycomb complex protein BMI-1 (BMI1) is a ~36 kDa protein. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2.

1. Wang, H. et al. (2004) Nature 431, 873-8. PMID: 15386022

2. Cao, R. et al. (2005) Molecular cell 20, 845-54. PMID: 16359901

3. Li, Z. et al. (2006) The Journal of biological chemistry 281, 20643-9. PMID: 16714294

4. Bentley, ML. et al. (2011) The EMBO journal 30, 3285-97. PMID: 21772249

5. McGinty, RK. et al. (2014) Nature 514, 591-6. PMID: 25355358

6. Taherbhoy, AM. et al. (2015) Nature communications 6, 7621. PMID: 26151332

7. Gray, F. et al. (2016) Nature communications 7, 13343. PMID: 27827373

Note

For research use only.

Images

References

Datasheet

Anti-BMI1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-BMI1 Polyclonal Antibody (HW616014)

Overview

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References

Datasheet

SDS

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