Anti-BRCC3 Polyclonal Antibody (HA695014)

Name: Anti-BRCC3 Polyclonal Antibody
Brand: abinScience
SKU: HA695014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HA695014

Description

Anti-BRCC3 Polyclonal Antibody (HA695014) is a rabbit polyclonal antibody detecting Lys-63-specific deubiquitinase BRCC36; 3.4.19.-; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36; BRCC3; BRCC36; C6.1A; CXorf53 in ELISA, IHC, WB. Suitable for Human and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant human BRCC3 (Val12-Thr223).

Target

Lys-63-specific deubiquitinase BRCC36; 3.4.19.-; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRISC complex subunit BRCC36; BRCC3; BRCC36; C6.1A; CXorf53

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P46736, A5PJP6

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Lys-63-specific deubiquitinase BRCC36 is a ~36 kDa protein. Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates.

1. Cooper, EM. et al. (2009) The EMBO journal 28, 621-31. PMID: 19214193

2. Feng, L. et al. (2010) The Journal of biological chemistry 285, 30982-8. PMID: 20656690

3. Zheng, H. et al. (2013) Cell reports 5, 180-93. PMID: 24075985

4. Zeqiraj, E. et al. (2015) Molecular cell 59, 970-83. PMID: 26344097

5. Dong, Y. et al. (2003) Molecular cell 12, 1087-99. PMID: 14636569

6. Chen, X. et al. (2006) Cancer research 66, 5039-46. PMID: 16707425

7. Sobhian, B. et al. (2007) Science (New York, N.Y.) 316, 1198-202. PMID: 17525341

9. Shao, G. et al. (2009) Genes & development 23, 740-54. PMID: 19261746

10. Feng, L. et al. (2009) Genes & development 23, 719-28. PMID: 19261748

Note

For research use only

Images

References

Datasheet

Anti-BRCC3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-BRCC3 Polyclonal Antibody (HA695014)

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Datasheet

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