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Anti-BRDT Polyclonal Antibody (HA831014)

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Overview
Catalog No.HA831014
Description
Anti-BRDT Polyclonal Antibody (HA831014) is a rabbit polyclonal antibody detecting Bromodomain testis-specific protein; Cancer/testis antigen 9; CT9; RING3-like protein; BRDT in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human BRDT (Val269-Pro377).
Target Bromodomain testis-specific protein; Cancer/testis antigen 9; CT9; RING3-like protein; BRDT
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q58F21, D4A7T3, Q91Y44
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Bromodomain testis-specific protein (BRDT) is a ~107 kDa protein. Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA. Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids.

1. Filippakopoulos, P. et al. (2012) Cell 149, 214-31. PMID: 22464331
2. Matzuk, MM. et al. (2012) Cell 150, 673-84. PMID: 22901802
Note For research use only
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Formula
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Anti-BRDT Polyclonal Antibody

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