Anti-C5 Polyclonal Antibody (HF687014)

Name: Anti-C5 Polyclonal Antibody
Brand: abinScience
SKU: HF687014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF687014

Description

Anti-C5 Polyclonal Antibody (HF687014) is a rabbit polyclonal antibody detecting C5 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human C5 (Thr678-Arg751).

Target

CPAMD4, C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, Complement C5

Purification

Purified by antigen affinity column.

Accession

P01031

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Complement C5 is a ~188 kDa protein. Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Activated downstream of classical, alternative, lectin and GZMK complement pathways.

1. Rawal, N. et al. (2003) The Journal of biological chemistry 278, 38476-83. PMID: 12878586

2. Rawal, N. et al. (2008) The Journal of biological chemistry 283, 7853-63. PMID: 18204047

3. Heesterbeek, DA. et al. (2019) The EMBO journal 38. PMID: 30643019

4. DiScipio, RG. et al. (1983) The Journal of biological chemistry 258, 10629-36. PMID: 6554279

5. Donado, CA. et al. (2025) Nature 641, 211-221. PMID: 39914456

6. Lan, F. et al. (2025) Nature 638, 490-498. PMID: 39814882

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-C5 polyclonal antibody at 1ug/mL on recombinant C5 (Catalog No. HF687012).

References

Datasheet

Anti-C5 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-C5 Polyclonal Antibody (HF687014)

Overview

Images

References

Datasheet

SDS

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