Anti-C8B Polyclonal Antibody (HY246014)

Name: Anti-C8B Polyclonal Antibody
Brand: abinScience
SKU: HY246014
Price: 168 USD
Availability: InStock

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Overview
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SDS

Overview

Catalog No.

HY246014

Description

Anti-C8B Polyclonal Antibody (HY246014) is a rabbit polyclonal antibody detecting C8B in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human C8B (Cys162-His504).

Target

C8B, Complement component C8 beta chain, Complement component 8 subunit beta

Purification

Purified by antigen affinity column.

Accession

P07358

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Complement component C8 beta chain (C8B) is a ~66 kDa protein. Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C8B, together with C8A and C8G, inserts into the target membrane, but does not form pores by itself. During MAC assembly, associates with C5b, C6 and C7 to form the C5b8 intermediate complex that inserts into the target membrane and traverses the bilayer increasing membrane rigidity.

1. Hadders, MA. et al. (2012) Cell reports 1, 200-7. PMID: 22832194

2. Serna, M. et al. (2016) Nature communications 7, 10587. PMID: 26841837

3. Sharp, TH. et al. (2016) Cell reports 15, 1-8. PMID: 27052168

4. Menny, A. et al. (2018) Nature communications 9, 5316. PMID: 30552328

5. Steckel, EW. et al. (1980) The Journal of biological chemistry 255, 11997-2005. PMID: 7440581

6. Donado, CA. et al. (2025) Nature 641, 211-221. PMID: 39914456

7. Lan, F. et al. (2025) Nature 638, 490-498. PMID: 39814882

8. Steckel, EW. et al. (1983) The Journal of biological chemistry 258, 4318-24. PMID: 6833260

Note

For research use only.

Images

References

Datasheet

Anti-C8B Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-C8B Polyclonal Antibody (HY246014)

Overview

Images

References

Datasheet

SDS

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