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Anti-CA1 Polyclonal Antibody (HF860014)

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Overview
Catalog No.HF860014
Description
Anti-CA1 Polyclonal Antibody (HF860014) is a rabbit polyclonal antibody detecting CA1 in ELISA, IHC, WB. Suitable for Human, Macaca mulatta, Macaca nemestrina, and Pan troglodytes.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CA1 (Ala2-Phe261).
Target CA-I, Carbonate dehydratase I, Carbonic anhydrase B, CA1, CAB, Carbonic anhydrase 1, Carbonic anhydrase I
Purification Purified by antigen affinity column.
Accession P00915
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Carbonic anhydrase 1 (CA1) is a ~28 kDa protein. Catalyzes the reversible hydration of carbon dioxide. Can hydrate cyanamide to urea.

1. Briganti, F. et al. (1999) Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 4, 528-36. PMID: 10550681
2. Jude, KM. et al. (2006) Journal of the American Chemical Society 128, 3011-8. PMID: 16506782
3. Temperini, C. et al. (2006) Journal of medicinal chemistry 49, 3019-27. PMID: 16686544
4. Temperini, C. et al. (2006) Chemistry (Weinheim an der Bergstrasse, Germany) 12, 7057-66. PMID: 16807956
5. Temperini, C. et al. (2007) Bioorganic & medicinal chemistry letters 17, 628-35. PMID: 17127057
6. Temperini, C. et al. (2007) Bioorganic & medicinal chemistry letters 17, 2210-5. PMID: 17314045
7. Srivastava, DK. et al. (2007) Journal of the American Chemical Society 129, 5528-37. PMID: 17407288
8. Di Fiore, A. et al. (2009) Proteins 74, 164-75. PMID: 18618712
9. Crocetti, L. et al. (2009) Bioorganic & medicinal chemistry letters 19, 1371-5. PMID: 19186056
10. Maresca, A. et al. (2009) Journal of the American Chemical Society 131, 3057-62. PMID: 19206230
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-CA1 Polyclonal Antibody

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