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Anti-CALCA/Calcitonin Polyclonal Antibody (HF635014)

Anti-CALCA/Calcitonin Polyclonal Antibody
Anti-CALCA/Calcitonin Polyclonal Antibody
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Overview
Catalog No.HF635014
Description
Anti-CALCA/Calcitonin Polyclonal Antibody (HF635014) is a rabbit polyclonal antibody detecting Calcitonin in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CALCA/Calcitonin (Ala26-Asn141).
Target CCP, PDN-21, CALCA, Calcitonin carboxyl-terminal peptide, Calcitonin, CALC1
Purification Purified by antigen affinity column.
Accession P01258
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Calcitonin (CALCA) is a ~15 kDa protein. Calcitonin is a peptide hormone that causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Calcitonin function is mediated by the calcitonin receptor/CALCR and the CALCR-RAMP2 (AMYR2) receptor complex.

1. Cao, J. et al. (2022) Science (New York, N.Y.) 375, eabm9609. PMID: 35324283
Note For research use only.
Images
  • Anti-CALCA/Calcitonin Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CALCA/Calcitonin polyclonal antibody at 1ug/mL on recombinant CALCA/Calcitonin (Catalog No. HF635012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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