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Anti-CALML3 Polyclonal Antibody (HB757014)

Anti-CALML3 Polyclonal Antibody
Anti-CALML3 Polyclonal Antibody
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Overview
Catalog No.HB757014
Description
Anti-CALML3 Polyclonal Antibody (HB757014) is a rabbit polyclonal antibody detecting CALML3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Aplysia californica, and Arbacia punctulata.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CALML3 (Met1-Lys149).
Target CALML3, CaM-like protein, Calmodulin-related protein NB-1, Calmodulin-like protein 3, CLP
Purification Purified by antigen affinity column.
Accession P27482
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Calmodulin-like protein 3 (CALML3) is a ~16 kDa protein. May function as a specific light chain of unconventional myosin-10 (MYO10), also enhances MYO10 translation, possibly by acting as a chaperone for the emerging MYO10 heavy chain protein. May compete with calmodulin by binding, with different affinities, to cellular substrates.

Note For research use only.
Images
  • Anti-CALML3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CALML3 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: A431 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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