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Anti-CALU Polyclonal Antibody (HT280014)

Anti-CALU Polyclonal Antibody
Anti-CALU Polyclonal Antibody
Anti-CALU Polyclonal Antibody
Anti-CALU Polyclonal Antibody
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  • Overview

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  • Datasheet

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Overview
Catalog No.HT280014
Description
Anti-CALU Polyclonal Antibody (HT280014) is a rabbit polyclonal antibody detecting CALU in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CALU (Lys20-Phe315).
Target Crocalbin, CALU, Calumenin, IEF SSP 9302
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O43852
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Calumenin is a ~37 kDa protein. Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity.

Note For research use only.
Images
  • Anti-CALU Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CALU polyclonal antibody at 1ug/mL on recombinant CALU (Catalog No. HT280012).

  • Anti-CALU Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CALU polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: K562 cell lysate
    Lane 3: Hela cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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