Anti-CANT1 Polyclonal Antibody (HV161014)

Name: Anti-CANT1 Polyclonal Antibody
Brand: abinScience
SKU: HV161014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HV161014

Description

Anti-CANT1 Polyclonal Antibody (HV161014) is a rabbit polyclonal antibody detecting CANT1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human CANT1 (Ile107-Phe400).

Target

CANT1, Putative NF-kappa-B-activating protein 107, Apyrase homolog, Soluble calcium-activated nucleotidase 1, SCAN-1, SHAPY, Putative MAPK-activating protein PM09

Purification

Purified by antigen affinity column.

Accession

Q8WVQ1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Soluble calcium-activated nucleotidase 1 (CANT1) is a ~44 kDa protein. Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP. Involved in proteoglycan synthesis.

1. Smith, TM. et al. (2002) Archives of biochemistry and biophysics 406, 105-15. PMID: 12234496

2. Dai, J. et al. (2004) Cell 116, 649-59. PMID: 15006348

3. Yang, M. et al. (2004) Biochemistry 43, 9185-94. PMID: 15248776

4. Yang, M. et al. (2006) The Journal of biological chemistry 281, 28307-17. PMID: 16835225

5. Nizon, M. et al. (2012) Human mutation 33, 1261-6. PMID: 22539336

Note

For research use only.

Images

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CANT1 (HV161014) at a concentration of 26 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CANT1 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

References

Datasheet

Anti-CANT1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CANT1 Polyclonal Antibody (HV161014)

Overview

Images

References

Datasheet

SDS

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