Anti-CAPN1 Polyclonal Antibody (HC129014)

Name: Anti-CAPN1 Polyclonal Antibody
Brand: abinScience
SKU: HC129014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC129014

Description

Anti-CAPN1 Polyclonal Antibody (HC129014) is a rabbit polyclonal antibody detecting CAPN1 in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant human CAPN1 (Gly27-Ser360).

Target

Calpain 1, CANP 1, CANPL1, CAPN1, Calcium-activated neutral proteinase 1, Calpain mu-type, Calpain-1 catalytic subunit, Calpain-1 large subunit, Cell proliferation-inducing gene 30 protein, EC:3.4.22.52, Micromolar-calpain, muCANP

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P07384, P97571, O35350

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Calpain-1 catalytic subunit (CAPN1) is a ~81 kDa protein. Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-375', 'Gly-387' and 'His-409'. Cleaves and activates caspase-7 (CASP7).

1. Gafni, J. et al. (2009) The Journal of biological chemistry 284, 25441-9. PMID: 19617626

2. Hsu, CY. et al. (2011) The Journal of biological chemistry 286, 23222-33. PMID: 21531719

3. Sorimachi, H. et al. (1990) Biological chemistry Hoppe-Seyler 371 Suppl, 171-6. PMID: 2400579

4. Ono, Y. et al. (2013) Journal of molecular biology 425, 2955-72. PMID: 23707407

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-CAPN1 polyclonal antibody at 1ug/mL on recombinant CAPN1 (Catalog No. HC129012).

References

Datasheet

Anti-CAPN1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CAPN1 Polyclonal Antibody (HC129014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us