Anti-CAPN2 Polyclonal Antibody (HB291014)

Name: Anti-CAPN2 Polyclonal Antibody
Brand: abinScience
SKU: HB291014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB291014

Description

Anti-CAPN2 Polyclonal Antibody (HB291014) is a rabbit polyclonal antibody detecting CAPN2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CAPN2 (Leu45-Asp514).

Target

Calpain M-type, Calcium-activated neutral proteinase 2, CANPL2, Calpain-2 catalytic subunit, CAPN2, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CANP 2

Purification

Purified by antigen affinity column.

Accession

P17655

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Calpain-2 catalytic subunit (CAPN2) is a ~79 kDa protein. Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs.

1. Sánchez-Sánchez, F. et al. (2007) The Journal of biological chemistry 282, 27810-24. PMID: 17650508

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CAPN2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: MDA-MB-231 cell lysate
Lane 2: A549 cell lysate
Lane 3: K562 cell lysate
Lane 4: C2C12 cell lysate
Lane 5: PC-3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: U2OS cell lysate

References

Datasheet

Anti-CAPN2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CAPN2 Polyclonal Antibody (HB291014)

Overview

Images

References

Datasheet

SDS

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