Anti-Capsid Polyclonal Antibody (VK719014) is a rabbit polyclonal antibody detecting Capsid protein C in ELISA, IHC, WB. Suitable for Dengue virus type 1 and Dengue virus type 3.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
Species reactivity
Dengue virus 1 (DENV-1)
Applications
ELISA, IHC, WB
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Capsid (Asn21-Arg100).
Target
Genome polyprotein, Capsid protein C, Capsid protein, Core protein, Protein prM, Precursor membrane protein, Peptide pr, Peptide precursor, Small envelope protein M, Matrix protein, Envelope protein E, Non-structural protein 1, NS1, Non-structural protein 2A, NS2A, Serine protease subunit NS2B, Flavivirin protease NS2B regulatory subunit, Non-structural protein 2B, Serine protease NS3, EC:3.4.21.91, EC:3.6.1.15, EC:3.6.4.13, Flavivirin protease NS3 catalytic subunit, Non-structural protein 3, Non-structural protein 4A, NS4A, Peptide 2k, Non-structural protein 4B, NS4B, RNA-directed RNA polymerase NS5, EC:2.1.1.56, EC:2.1.1.57, EC:2.7.7.48, Non-structural protein 5
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application
Dilution
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background
Genome polyprotein (Capsid) is a ~378 kDa protein. Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (Probable). Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions.
2. Sangiambut, S. et al. (2008) The Journal of general virology 89, 1254-1264. PMID: 18420804
3. Colpitts, TM. et al. (2011) PloS one 6, e24365. PMID: 21909430
4. Bhuvanakantham, R. et al. (2013) Cellular microbiology 15, 1688-706. PMID: 23522008
Note
For research use only
Images
Western blot
Western blot analysis was performed using anti-NENV-1 Capsid polyclonal antibody at 1ug/mL on various samples. Lane 1: recombinant DENV-1 Capsid protein C/Core protein (Catalog No: VK719012)
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