Anti-CASP8 Polyclonal Antibody (HC266014)

Name: Anti-CASP8 Polyclonal Antibody
Brand: abinScience
SKU: HC266014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC266014

Description

Anti-CASP8 Polyclonal Antibody (HC266014) is a rabbit polyclonal antibody detecting CASP8 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CASP8 (Ser217-Asp479).

Target

CAP4, MCH5, FADD-homologous ICE/ced-3-like protease, FLICE, Apoptotic cysteine protease, MORT1-associated ced-3 homolog, MACH, FADD-like ICE, ICE-like apoptotic protease 5, CASP8, CASP-8, Caspase-8, Apoptotic protease Mch-5

Purification

Purified by antigen affinity column.

Accession

Q14790

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Caspase-8 (CASP8) is a ~55 kDa protein. Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. Initiator protease that induces extrinsic apoptosis by mediating cleavage and activation of effector caspases responsible for FAS/CD95-mediated and TNFRSF1A-induced cell death. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. Binding to the adapter molecule FADD recruits it to either receptor FAS/TNFRSF6 or TNFRSF1A. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation.

1. Blasche, S. et al. (2013) PloS one 8, e58937. PMID: 23516580

2. Peng, T. et al. (2022) Molecular cell 82, 1806-1820.e8. PMID: 35338844

3. Liu, Y. et al. (2022) mBio 13, e0069022. PMID: 35446120

4. Boldin, MP. et al. (1996) Cell 85, 803-15. PMID: 8681376

5. Muzio, M. et al. (1996) Cell 85, 817-27. PMID: 8681377

6. Srinivasula, SM. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 14486-91. PMID: 8962078

7. Muzio, M. et al. (1997) The Journal of biological chemistry 272, 2952-6. PMID: 9006941

8. Medema, JP. et al. (1997) The EMBO journal 16, 2794-804. PMID: 9184224

9. Denault, JB. et al. (2006) Molecular cell 23, 523-33. PMID: 16916640

10. Tao, P. et al. (2020) Nature 577, 109-114. PMID: 31827280

Note

For research use only.

Images

References

Datasheet

Anti-CASP8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CASP8 Polyclonal Antibody (HC266014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us