Anti-CBR3 Polyclonal Antibody (HF500014)

Name: Anti-CBR3 Polyclonal Antibody
Brand: abinScience
SKU: HF500014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF500014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CBR3 (Met1-Trp277).

Target

Carbonyl reductase [NADPH] 3, Short chain dehydrogenase/reductase family 21C member 2, NADPH-dependent carbonyl reductase 3, CBR3

Purification

Purified by antigen affinity column.

Accession

O75828

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Carbonyl reductase [NADPH] 3 (CBR3) is a ~30 kDa protein. Catalyzes the NADPH-dependent reduction of carbonyl compounds to their corresponding alcohols. Has low NADPH-dependent oxidoreductase activity. Acts on several orthoquinones, acts as well on non-quinone compounds, such as isatin or on the anticancer drug oracin. Best substrates for CBR3 is 1,2- naphthoquinone, hence could play a role in protection against cytotoxicity of exogenous quinones. Exerts activity toward ortho-quinones but not paraquinones.

1. Miura, T. et al. (2008) Molecular and cellular biochemistry 315, 113-21. PMID: 18493841

2. Lakhman, SS. et al. (2005) Drug metabolism and disposition: the biological fate of chemicals 33, 254-7. PMID: 15537833

3. Pilka, ES. et al. (2009) PloS one 4, e7113. PMID: 19841672

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CBR3 polyclonal antibody at 1μg/ml on various samples.
Lane 1: MCF7 cell lysate
Lane 2: Mouse ovary lysate
Lane 3: Mouse uterus lysate

References

Datasheet

Anti-CBR3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CBR3 Polyclonal Antibody (HF500014)

Overview

Images

References

Datasheet

SDS

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