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Anti-CCHFV Nucleoprotein Polyclonal Antibody (VK608024)

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Overview
Catalog No.VK608024
Description
Anti-CCHFV Nucleoprotein Polyclonal Antibody (VK608024) is a rabbit polyclonal antibody detecting nucleoprotein in ELISA, IHC, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityCrimean-Congo hemorrhagic fever virus (CCHFV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant CCHFV Nucleoprotein (Met1-Ile482).
Target nucleoprotein, nucleoprotein
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P27317, P89522
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nucleoprotein is a ~53 kDa protein. Binds dsRNA and ssRNA and probably participates in the packaging of viral genome. In the dsRNA binding mode, the nucleocapsid protein specifically binds to the vRNA panhandle secondary structure formed at the termini of viral genome. Does not discriminate between viral and nonviral RNAs through ssRNA binding mode. Displays dsDNA endonuclease activity that is sequence non-specific.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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