Anti-CCT5 Polyclonal Antibody (HW730014)

Name: Anti-CCT5 Polyclonal Antibody
Brand: abinScience
SKU: HW730014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW730014

Description

Anti-CCT5 Polyclonal Antibody (HW730014) is a rabbit polyclonal antibody detecting CCT5 in ELISA, IHC, WB. Suitable for Mouse, Rat, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CCT5 (Met1-Glu541).

Target

CCT-epsilon, CCT5, CCTE, Chaperonin containing T-complex polypeptide 1 subunit 5, EC:3.6.1.-, KIAA0098, T-complex protein 1 subunit epsilon, TCP-1-epsilon

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P48643

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

P48643

Background

T-complex protein 1 subunit epsilon (CCT5) is a ~59 kDa protein. Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia.

1. Freund, A. et al. (2014) Cell 159, 1389-403. PMID: 25467444

2. Gestaut, D. et al. (2022) Cell 185, 4770-4787.e20. PMID: 36493755

3. Kelly, JJ. et al. (2022) Nature structural & molecular biology 29, 420-429. PMID: 35449234

4. Liu, C. et al. (2023) Communications biology 6, 531. PMID: 37193829

5. Seo, S. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 1488-93. PMID: 20080638

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-CCT5 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: A431 cell lysate
Lane 3: 293T cell lysate
Lane 4: MOLT-4 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: HepG2 cell lysate
Lane 7: NIH3T3 cell lysate
Lane 8: A549 cell lysate

References

Datasheet

Anti-CCT5 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CCT5 Polyclonal Antibody (HW730014)

Overview

Images

References

Datasheet

SDS

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