Anti-CD106/VCAM1 Polyclonal Antibody (HB032014)

Name: Anti-CD106/VCAM1 Polyclonal Antibody
Brand: abinScience
SKU: HB032014
Price: 168 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB032014

Description

Anti-CD106/VCAM1 Polyclonal Antibody (HB032014) is a rabbit polyclonal antibody detecting VCAM1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD106/VCAM1 (Phe25-Glu698).

Target

Vascular cell adhesion protein 1, VCAM1, CD106, INCAM-100, V-CAM 1, VCAM-1

Purification

Purified by antigen affinity column.

Accession

P19320

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Vascular cell adhesion protein 1 (CD106/VCAM1) is a ~81 kDa protein. Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation.

1. Li, Y. et al. (2020) Journal of molecular cell biology 12, 190-201. PMID: 31310649

2. Taooka, Y. et al. (1999) The Journal of cell biology 145, 413-20. PMID: 10209034

3. Marchese, ME. et al. (2012) Biochemistry 51, 8235-46. PMID: 22970700

4. Pinho, S. et al. (2022) Nature cell biology 24, 290-298. PMID: 35210567

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD106/VCAM1 polyclonal antibody at 1ug/mL on recombinant CD106/VCAM1 (Catalog No. HB032012).

References

Datasheet