Anti-CD119/IFNGR1 Polyclonal Antibody (HB990014)

Name: Anti-CD119/IFNGR1 Polyclonal Antibody
Brand: abinScience
SKU: HB990014
Price: 168 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HB990014

Description

Anti-CD119/IFNGR1 Polyclonal Antibody (HB990014) is a rabbit polyclonal antibody detecting IFNGR1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD119/IFNGR1 (Glu18-Gly245).

Target

IFN-gamma-R1, Interferon gamma receptor 1, CDw119, Interferon gamma receptor alpha-chain, IFN-gamma receptor 1, IFNGR1, IFN-gamma-R-alpha, CD119

Purification

Purified by antigen affinity column.

Accession

P15260

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interferon gamma receptor 1 (CD119/IFNGR1) is a ~54 kDa protein. Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Associates with transmembrane accessory factor IFNGR2 to form a functional receptor. Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to dimerization, translocation to the nucleus, and stimulation of target gene transcription.

1. van de Wetering, D. et al. (2010) Molecular immunology 47, 1023-30. PMID: 20015550

2. Thiel, DJ. et al. (2000) Structure (London, England : 1993) 8, 927-36. PMID: 10986460

3. Aguet, M. et al. (1988) Cell 55, 273-80. PMID: 2971451

4. Sakatsume, M. et al. (1995) The Journal of biological chemistry 270, 17528-34. PMID: 7615558

5. Walter, MR. et al. (1995) Nature 376, 230-5. PMID: 7617032

6. Kotenko, SV. et al. (1995) The Journal of biological chemistry 270, 20915-21. PMID: 7673114

7. Londino, JD. et al. (2017) The Biochemical journal 474, 3543-3557. PMID: 28883123

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD119/IFNGR1 polyclonal antibody at 1ug/mL on recombinant CD119/IFNGR1 (Catalog No. HB990012).

References

Datasheet

Anti-CD119/IFNGR1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CD119/IFNGR1 Polyclonal Antibody (HB990014)

Overview

Images

References

Datasheet

SDS

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