Anti-CD126/IL6R/IL-6RA Polyclonal Antibody (HY583014)

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50ug
100ug

Number:

Overview
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References
Datasheet

Overview

Catalog No.

HY583014

Description

Anti-CD126/IL6R/IL-6RA Polyclonal Antibody (HY583014) is a rabbit polyclonal antibody detecting IL-6RA in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD126/IL6R/IL-6RA (Met1-Pro365).

Target

sIL6R, Interleukin-6 receptor subunit alpha, CD126, IL-6R 1, IL-6R subunit alpha, Membrane glycoprotein 80, gp80, IL6R, IL-6RA, IL-6 receptor subunit alpha, IL-6R-alpha

Purification

Purified by antigen affinity column.

Accession

P08887

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-6 receptor subunit alpha (CD126/IL6R) is a ~51 kDa protein. Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system.

1. Larsen, JV. et al. (2017) Molecular and cellular biology 37. PMID: 28265003

2. Kang, S. et al. (2019) Immunity 50, 1007-1023. PMID: 30995492

3. Spencer, S. et al. (2019) The Journal of experimental medicine 216, 1986-1998. PMID: 31235509

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD126/IL6R/IL-6RA polyclonal antibody at 1ug/mL on recombinant CD126/IL6R/IL-6RA (Catalog No. HY583012).

References

Datasheet