Anti-CD14 Polyclonal Antibody (HY038014)

Name: Anti-CD14 Polyclonal Antibody
Brand: abinScience
SKU: HY038014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY038014

Description

Anti-CD14 Polyclonal Antibody (HY038014) is a rabbit polyclonal antibody detecting CD14 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD14 (Thr20-Leu368).

Target

Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14

Purification

Purified by antigen affinity column.

Accession

P08571

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Monocyte differentiation antigen CD14 is a ~40 kDa protein. Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-).

1. Wright, SD. et al. (1990) Science (New York, N.Y.) 249, 1431-3. PMID: 1698311

2. Kelley, SL. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 190, 1304-11. PMID: 23264655

3. Tsukamoto, H. et al. (2010) International immunology 22, 271-80. PMID: 20133493

4. Murakami, K. et al. (2012) FEBS letters 586, 319-24. PMID: 22265692

5. Haziot, A. et al. (1996) Immunity 4, 407-14. PMID: 8612135

6. Triantafilou, M. et al. (2006) The Journal of biological chemistry 281, 31002-11. PMID: 16880211

7. Estruch, M. et al. (2013) Atherosclerosis 229, 356-62. PMID: 23880187

Note

For research use only.

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References

Datasheet