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Anti-CD170/SIGLEC5 Polyclonal Antibody (HT187014)

Anti-CD170/SIGLEC5 Polyclonal Antibody
Anti-CD170/SIGLEC5 Polyclonal Antibody
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Spec:
  • 50ug
  • 100ug
Number:
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Overview
Catalog No.HT187014
Description
Anti-CD170/SIGLEC5 Polyclonal Antibody (HT187014) is a rabbit polyclonal antibody detecting SIGLEC5 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CD170/SIGLEC5 (Phe458-Lys551).
Target CD33 antigen-like 2, Obesity-binding protein 2, OBBP2, Siglec-5, CD170, OB-binding protein 2, Sialic acid-binding Ig-like lectin 5, OB-BP2, SIGLEC5, CD33L2
Purification Purified by antigen affinity column.
Accession O15389
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Sialic acid-binding Ig-like lectin 5 (CD170/SIGLEC5) is a ~60 kDa protein. Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Note For research use only.
Images
  • Anti-CD170/SIGLEC5 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CD170/SIGLEC5 polyclonal antibody at 1ug/mL on recombinant CD170/SIGLEC5 (Catalog No. HT187012).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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