Anti-Human Band 3 Polyclonal Antibody (HF626014)

Name: Anti-Human Band 3 Polyclonal Antibody
Brand: abinScience
SKU: HF626014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF626014

Description

Anti-Human Band 3 Polyclonal Antibody (HF626014) is a rabbit polyclonal antibody detecting Band 3 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant CD233/SLC4A1 (Ala35-Ser290).

Target

AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, Band 3 anion transport protein, CD233, DI, EPB3, SLC4A1, Solute carrier family 4 member 1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

P02730

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Band 3 anion transport protein (CD233/SLC4A1) is a ~101 kDa protein. Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Component of the ankyrin-1 complex of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.

1. Bruce, LJ. et al. (2000) The Biochemical journal 350 Pt 1, 41-51. PMID: 10926824

2. Rungroj, N. et al. (2004) The Journal of biological chemistry 279, 13833-8. PMID: 14734552

3. Schofield, AE. et al. (1992) Journal of molecular biology 223, 949-58. PMID: 1538405

4. Bruce, LJ. et al. (2005) Nature genetics 37, 1258-63. PMID: 16227998

5. Ungsupravate, D. et al. (2010) Molecular membrane biology 27, 92-103. PMID: 20151848

6. Shnitsar, V. et al. (2013) The Journal of biological chemistry 288, 33848-33860. PMID: 24121512

7. Genetet, S. et al. (2017) Scientific reports 7, 46170. PMID: 28387307

8. Vallese, F. et al. (2022) Nature structural & molecular biology 29, 706-718. PMID: 35835865

Note

For research use only.

Images

References

Datasheet

Anti-Human Band 3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human Band 3 Polyclonal Antibody (HF626014)

Overview

Images

References

Datasheet

SDS