Anti-CD247/CD3Z Polyclonal Antibody (HB150014)

Name: Anti-CD247/CD3Z Polyclonal Antibody
Brand: abinScience
SKU: HB150014
Price: 96 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HB150014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD247/CD3Z (Arg52-Arg164).

Target

CD3Z, CD247, TCRZ, T-cell receptor T3 zeta chain, T3Z, T-cell surface glycoprotein CD3 zeta chain

Purification

Purified by antigen affinity column.

Accession

P20963

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

T-cell surface glycoprotein CD3 zeta chain (CD247/CD3Z) is a ~18 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD247/CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme.

1. Burgess, KE. et al. (1992) Proceedings of the National Academy of Sciences of the United States of America 89, 9311-5. PMID: 1384049

2. Osman, N. et al. (1992) European journal of immunology 22, 2995-3000. PMID: 1385158

3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098

4. Iwashima, M. et al. (1994) Science (New York, N.Y.) 263, 1136-9. PMID: 7509083

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD247 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate

References

Datasheet

Anti-CD247/CD3Z Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-CD247/CD3Z Polyclonal Antibody (HB150014)

Overview

Images

References

Datasheet

SDS

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