Anti-CD324/CDH1 Polyclonal Antibody (HY460014)

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50ug
100ug

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Overview
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Datasheet

Overview

Catalog No.

HY460014

Description

Anti-CD324/CDH1 Polyclonal Antibody (HY460014) is a rabbit polyclonal antibody detecting CD324 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Dog, Pongo abelii

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD324/CDH1 (Asp155-Asp367).

Target

CD324, Uvomorulin, Epithelial cadherin, E-cadherin, CDHE, CAM 120/80, UVO, CDH1, Cadherin-1

Purification

Purified by antigen affinity column.

Accession

P12830

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cadherin-1 (CD324/CDH1) is a ~97 kDa protein. Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane. Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques.

1. Meigs, TE. et al. (2002) The Journal of biological chemistry 277, 24594-600. PMID: 11976333

2. Shafraz, O. et al. (2018) eLife 7. PMID: 29999492

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD324/CDH1 polyclonal antibody at 1ug/mL on recombinant CD324/CDH1 (Catalog No. HY460012).

References

Datasheet