Anti-CD47 Polyclonal Antibody (HC359014)

Name: Anti-CD47 Polyclonal Antibody
Brand: abinScience
SKU: HC359014
Price: 168 USD
Availability: InStock

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Overview
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Overview

Catalog No.

HC359014

Description

Anti-CD47 Polyclonal Antibody (HC359014) is a rabbit polyclonal antibody detecting CD47 in ELISA, IHC, WB. Suitable for Human and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD47 (Gln19-Pro139).

Target

Leukocyte surface antigen CD47, CD47, Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Protein MER6, MER6

Purification

Purified by antigen affinity column.

Accession

Q08722

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Leukocyte surface antigen CD47 (MER6) is a ~35 kDa protein. Adhesive protein that mediates cell-to-cell interactions. Acts as a receptor for thrombospondin THBS1 and as modulator of integrin signaling through the activation of heterotrimeric G proteins. Involved in signal transduction, cardiovascular homeostasis, inflammation, apoptosis, angiogenesis, cellular self-renewal, and immunoregulation. Plays a role in modulating pulmonary endothelin EDN1 signaling. Modulates nitrous oxide (NO) signaling, in response to THBS1, hence playing a role as a pressor agent, supporting blood pressure. CD47 is the therapeutic target of magrolimab (clinical).

1. Latour, S. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 2547-54. PMID: 11509594

2. Piccio, L. et al. (2005) Blood 105, 2421-7. PMID: 15383453

3. Isenberg, JS. et al. (2009) The Journal of biological chemistry 284, 1116-25. PMID: 19004835

4. Lindberg, FP. et al. (1993) The Journal of cell biology 123, 485-96. PMID: 7691831

5. Gao, AG. et al. (1996) The Journal of biological chemistry 271, 21-4. PMID: 8550562

6. Rogers, NM. et al. (2017) Cardiovascular research 113, 15-29. PMID: 27742621

7. Ghimire, K. et al. (2020) Cells 9. PMID: 32679764

Note

For research use only.

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References

Datasheet