Anti-CD66a/CEACAM1 Polyclonal Antibody (HB880014)

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50ug
100ug

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Overview

Catalog No.

HB880014

Description

Anti-CD66a/CEACAM1 Polyclonal Antibody (HB880014) is a rabbit polyclonal antibody detecting CEACAM1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD66a/CEACAM1 (Gln35-Glu320).

Target

CD66a, BGP-1, Biliary glycoprotein 1, CEACAM1, Carcinoembryonic antigen-related cell adhesion molecule 1, BGP, BGP1

Purification

Purified by antigen affinity column.

Accession

P13688

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell adhesion molecule CEACAM1 (CD66a) is a ~57 kDa protein. Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner. Plays a role as coinhibitory receptor in immune response, insulin action and also functions as an activator during angiogenesis. Its coinhibitory receptor function is phosphorylation- and PTPN6 -dependent, which in turn, suppress signal transduction of associated receptors by dephosphorylation of their downstream effectors. Plays a role in immune response, of T cells, natural killer (NK) and neutrophils. Upon TCR/CD3 complex stimulation, inhibits TCR-mediated cytotoxicity by blocking granule exocytosis by mediating homophilic binding to adjacent cells, allowing interaction with and phosphorylation by LCK and interaction with the TCR/CD3 complex which recruits PTPN6 resulting in dephosphorylation of CD247 and ZAP70.

1. Chen, Z. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 6085-93. PMID: 18424730

2. Hosomi, S. et al. (2013) European journal of immunology 43, 2473-83. PMID: 23696226

3. Huang, YH. et al. (2015) Nature 517, 386-90. PMID: 25363763

4. Klaile, E. et al. (2005) Journal of cell science 118, 5513-24. PMID: 16291724

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD66a/CEACAM1 polyclonal antibody at 1ug/mL on recombinant CD66a/CEACAM1 (Catalog No. HB880012).

References

Datasheet