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Anti-CD85j/LILRB1 Polyclonal Antibody (HV631014)

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Overview
Catalog No.HV631014
Description
Anti-CD85j/LILRB1 Polyclonal Antibody (HV631014) is a rabbit polyclonal antibody detecting LILRB1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CD85j/LILRB1 (Ser223-His410).
Target LIR1, Immunoglobulin-like transcript 2, MIR7, CD85 antigen-like family member J, Monocyte/macrophage immunoglobulin-like receptor 7, ILT-2, Leukocyte immunoglobulin-like receptor subfamily B member 1, LIR-1, CD85j, ILT2, LILRB1, MIR-7, Leukocyte immunoglobulin-like receptor 1
Purification Purified by antigen affinity column.
Accession Q8NHL6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Leukocyte immunoglobulin-like receptor subfamily B member 1 (CD85j/LILRB1) is a ~70 kDa protein. Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Receptor for H301/UL18, a human cytomegalovirus class I MHC homolog. Ligand binding results in inhibitory signals and down-regulation of the immune response. Engagement of LILRB1 present on natural killer cells or T-cells by class I MHC molecules protects the target cells from lysis.

1. Shiroishi, M. et al. (2006) The Journal of biological chemistry 281, 10439-47. PMID: 16455647
2. Dulberger, CL. et al. (2017) Immunity 46, 1018-1029.e7. PMID: 28636952
3. Bellón, T. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 3351-9. PMID: 11907092
4. Cosman, D. et al. (1997) Immunity 7, 273-82. PMID: 9285411
5. Fanger, NA. et al. (1998) European journal of immunology 28, 3423-34. PMID: 9842885
6. Li, C. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 5767-72. PMID: 19304799
7. Fu, B. et al. (2017) Immunity 47, 1100-1113.e6. PMID: 29262349
8. Naji, A. et al. (2014) Journal of immunology (Baltimore, Md. : 1950) 192, 1536-46. PMID: 24453251
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-CD85j/LILRB1 Polyclonal Antibody

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