Anti-CDC20 Polyclonal Antibody (HC333014)

Name: Anti-CDC20 Polyclonal Antibody
Brand: abinScience
SKU: HC333014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HC333014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human CDC20 (Gln218-Arg471).

Target

Cell division cycle protein 20 homolog, p55CDC, CDC20

Purification

Purified by antigen affinity column.

Accession

Q12834

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell division cycle protein 20 homolog (CDC20) is a ~54 kDa protein. Substrate-specific adapter of the anaphase promoting complex/cyclosome (APC/C) complex that confers substrate specificity by binding to substrates and targeting them to the APC/C complex for ubiquitination and degradation. Recognizes and binds the destruction box (D box) on protein substrates. Involved in the metaphase/anaphase transition of cell cycle. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons.

1. Fang, G. et al. (1998) Molecular cell 2, 163-71. PMID: 9734353

2. Lin, Z. et al. (2015) Cell discovery 1, 15028-. PMID: 27030811

3. Choppara, S. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 998-1003. PMID: 29343641

4. Zhao, L. et al. (2020) Protein & cell 11, 921-927. PMID: 32666501

5. Kramer, ER. et al. (1998) Current biology : CB 8, 1207-10. PMID: 9811605

6. Fang, G. et al. (1998) Genes & development 12, 1871-83. PMID: 9637688

Note

For research use only.

Images

References

Datasheet

Anti-CDC20 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-CDC20 Polyclonal Antibody (HC333014)

Overview

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References

Datasheet

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