Anti-CDC23 Polyclonal Antibody (HP438014)

Name: Anti-CDC23 Polyclonal Antibody
Brand: abinScience
SKU: HP438014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP438014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human CDC23 (Gly366-Pro597).

Target

APC8, Anaphase-promoting complex subunit 8, CDC23, Cell division cycle protein 23 homolog, Cyclosome subunit 8, ANAPC8

Purification

Purified by antigen affinity column.

Accession

Q9UJX2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell division cycle protein 23 homolog (CDC23) is a ~68 kDa protein. Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins.

1. Jin, L. et al. (2008) Cell 133, 653-65. PMID: 18485873

2. Yau, RG. et al. (2017) Cell 171, 918-933.e20. PMID: 29033132

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CDC23 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate
Lane 2: Caco2 cell lysate

References

Datasheet

Anti-CDC23 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CDC23 Polyclonal Antibody (HP438014)

Overview

Images

References

Datasheet

SDS

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