Anti-CDC25B Polyclonal Antibody (HB248014)

Name: Anti-CDC25B Polyclonal Antibody
Brand: abinScience
SKU: HB248014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HB248014

Description

Anti-CDC25B Polyclonal Antibody (HB248014) is a rabbit polyclonal antibody detecting CDC25B in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CDC25B (Cys377-Gly566).

Target

Dual specificity phosphatase Cdc25B, CDC25B, CDC25HU2, M-phase inducer phosphatase 2

Purification

Purified by antigen affinity column.

Accession

P30305

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

M-phase inducer phosphatase 2 (CDC25B) is a ~64 kDa protein. Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. The three isoforms seem to have a different level of activity.

1. Galaktionov, K. et al. (1991) Cell 67, 1181-94. PMID: 1836978

2. Timofeev, O. et al. (2010) The Journal of biological chemistry 285, 16978-90. PMID: 20360007

3. Schmidt, A. et al. (2007) The EMBO journal 26, 1624-36. PMID: 17332740

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CDC25B polyclonal antibody at 1ug/mL on recombinant CDC25B (Catalog No. HB248012).

References

Datasheet

Anti-CDC25B Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CDC25B Polyclonal Antibody (HB248014)

Overview

Images

References

Datasheet

SDS

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