Anti-CDC27 Polyclonal Antibody (HB207014)

Name: Anti-CDC27 Polyclonal Antibody
Brand: abinScience
SKU: HB207014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HB207014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CDC27 (Asn17-Ser231).

Target

ANAPC3, Cell division cycle protein 27 homolog, CDC27, APC3, Anaphase-promoting complex subunit 3, CDC27Hs, D0S1430E, CDC27 homolog, D17S978E, H-NUC

Purification

Purified by antigen affinity column.

Accession

P30260

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cell division cycle protein 27 homolog (CDC27) is a ~91 kDa protein. Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins.

1. Jin, L. et al. (2008) Cell 133, 653-65. PMID: 18485873

2. Yau, RG. et al. (2017) Cell 171, 918-933.e20. PMID: 29033132

Note

For research use only.

Images

References

Datasheet

Anti-CDC27 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-CDC27 Polyclonal Antibody (HB207014)

Overview

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References

Datasheet

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