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Anti-CDK10 Polyclonal Antibody (HB532014)

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Overview
Catalog No.HB532014
Description
Anti-CDK10 Polyclonal Antibody (HB532014) is a rabbit polyclonal antibody detecting Cyclin-dependent kinase 10; 2.7.11.22; Cell division protein kinase 10; Serine/threonine-protein kinase PISSLRE; CDK10 in ELISA, IHC, WB. Suitable for Human, Rat, Mouse, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human CDK10 (Met1-Pro360).
Target Cyclin-dependent kinase 10; 2.7.11.22; Cell division protein kinase 10; Serine/threonine-protein kinase PISSLRE; CDK10
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q15131, Q4KM47, Q3UMM4, Q2TBL8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Cyclin-dependent kinase 10 (CDK10) is a ~41 kDa protein. Cyclin-dependent kinase that phosphorylates the transcription factor ETS2 (in vitro) and positively controls its proteasomal degradation (in cells). Involved in the regulation of actin cytoskeleton organization through the phosphorylation of actin dynamics regulators such as PKN2. Is a negative regulator of ciliogenesis through phosphorylation of PKN2 and promotion of RhoA signaling.

1. Guen, VJ. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 19525-30. PMID: 24218572
2. Guen, VJ. et al. (2016) Cell cycle (Georgetown, Tex.) 15, 678-88. PMID: 27104747
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-CDK10 Polyclonal Antibody

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