Anti-CDV F/Fusion glycoprotein F0 Polyclonal Antibody (VK648014)

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50ug
100ug

Number:

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK648014

Description

Anti-CDV F/Fusion glycoprotein F0 Polyclonal Antibody (VK648014) is a rabbit polyclonal antibody in WB, IHC, ELISA.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Canine distemper virus (CDV)

Applications

WB, IHC, ELISA

Host species

Rabbit

Isotype

IgG

Species

Canine distemper virus (strain Onderstepoort) (CDV)

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant CDV F/Fusion glycoprotein F0 (Gly246-Ser608).

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

P12569

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Fusion glycoprotein F0, Fusion glycoprotein F2, Fusion glycoprotein F1, F

Background

Fusion glycoprotein F0 is a ~72 kDa protein. Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CDV F/Fusion glycoprotein F0 polyclonal antibody at 1ug/mL on recombinant CDV F/Fusion glycoprotein F0 (Catalog No. VK648012).
Western blot

Western blot analysis was performed using anti-CDV-F polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant CDV F/Fusion glycoprotein F0 (Catalog No: VK648012)